Home > Publications by Willem Hanekom

Willem A Hanekom Deputy Director 2009 to 2013

  1. Analysis of host responses to Mycobacterium tuberculosis antigens in a multi-site study of subjects with different TB and HIV infection states in sub-Saharan Africa.
    Sutherland JS, Lalor MK, Black GF, Ambrose LR, Loxton AG, Chegou NN, Kassa D, Mihret A, Howe R, Mayanja-Kizza H, Gomez MP, Donkor S, Franken K, Hanekom W, Klein MR, Parida SK, Boom WH, Thiel BA, Crampin AC, Ota M, Walzl G, Ottenhoff TH, Dockrell HM, Kaufmann SH; GCGH Biomarkers for TB consortium.
    PLoS One. 2013 Sep 10;8(9):e74080. PMID: 24040170 PMCID: PMC3769366
  2. Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
    Dintwe OB, Day CL, Smit E, Nemes E, Gray C, Tameris M, McShane H, Mahomed H, Hanekom WA, Scriba TJ.
    Eur J Immunol. 2013 Sep;43(9):2409-20. PMID: 23737382 PMCID: PMC3816254
  3. Vaccine-induced immunity in early life.
    Kollmann TR, Levy O, Hanekom W.
    Vaccine. 2013 May 17;31(21):2481-2. PMID: 23588085 PMCID: PMC4922257
  4. TB incidence in an adolescent cohort in South Africa.
    Mahomed H, Ehrlich R, Hawkridge T, Hatherill M, Geiter L, Kafaar F, Abrahams DA, Mulenga H, Tameris M, Geldenhuys H, Hanekom WA, Verver S, Hussey GD.
    PLoS One. 2013;8(3):e59652. PMID: 23533639 PMCID: PMC3606161
  5. Lessons learnt from the first efficacy trial of a new infant tuberculosis vaccine since BCG.
    Tameris M, McShane H, McClain JB, Landry B, Lockhart S, Luabeya AK, Geldenhuys H, Shea J, Hussey G, van der Merwe L, de Kock M, Scriba T, Walker R, Hanekom W, Hatherill M, Mahomed H.
    Tuberculosis (Edinb). 2013 Mar;93(2):143-9. PMID: 23410889 PMCID: PMC3608032
  6. A strategy to determine HLA class II restriction broadly covering the DR, DP, and DQ allelic variants most commonly expressed in the general population.
    McKinney DM, Southwood S, Hinz D, Oseroff C, Arlehamn CS, Schulten V, Taplitz R, Broide D, Hanekom WA, Scriba TJ, Wood R, Alam R, Peters B, Sidney J, Sette A.
    Immunogenetics. 2013 May;65(5):357-70. PMID: 23392739 PMCID: PMC3633633
  7. Induction and Regulation of T-Cell Immunity by the Novel Tuberculosis Vaccine M72/AS01 in South African Adults
    Cheryl L. Day, Michele Tameris, Nazma Mansoor, Michele van Rooyen, Marwou de Kock, Hennie Geldenhuys, Mzwandile Erasmus, Lebohang Makhethe, E. Jane Hughes, Sebastian Gelderbloem, Anne Bollaerts, Patricia Bourguignon, Joe Cohen, Marie-Ange Demoitié, Pascal Mettens, Philippe Moris, Jerald C. Sadoff, Anthony Hawkridge, Gregory D. Hussey, Hassan Mahomed, Opokua Ofori-Anyinam, Willem A. Hanekom
    Am J Respir Crit Care Med. 2013 Aug 15; 188(4): 492–502. PMID: 23306546 PMCID: PMC3778736
  8. Longitudinal Changes in CD4+ T-Cell Memory Responses Induced by BCG Vaccination of Newborns
    Andreia P. Soares, Cheong K. C. Kwong Chung, Terry Choice, E. Jane Hughes, Gail Jacobs, Esme Janse van Rensburg, Gloria Khomba, Marwou de Kock, Lesedi Lerumo, Lebohang Makhethe, Mbulelo H. Maneli, Bernadette Pienaar, Erica Smit, Nontobeko G. Tena-Coki, Leandre van Wyk, W. Henry Boom, Gilla Kaplan, Thomas J. Scriba, Willem A. Hanekom
    J Infect Dis. 2013 Apr 1; 207(7): 1084–1094. PMID: 23293360 PMCID: PMC3583271
  9. Relationship between chemokine receptor expression, chemokine levels and HIV-1 replication in the lungs of persons exposed to Mycobacterium tuberculosis
    Barbara Kalsdorf, Keira H Skolimowska, Thomas J Scriba, Rod Dawson, Keertan Dheda, Kathryn Wood, Jessica Hofmeister, Willem A Hanekom, Christoph Lange, Robert J Wilkinson
    Eur J Immunol. 2013 Feb; 43(2): 540–549. PMID: 23147374 PMCID: PMC3791514
  10. Genetic Variation in TLR Genes in Ugandan and South African Populations and Comparison with HapMap Data
    Allison R. Baker, Feiyou Qiu, April Kaur Randhawa, David J. Horne, Mark D. Adams, Muki Shey, Jill Barnholtz-Sloan, Harriet Mayanja-Kizza, Gilla Kaplan, Willem A. Hanekom, W. Henry Boom, Thomas R. Hawn, Catherine M. Stein, Tuberculosis Research Unit (TBRU) and South African Tuberculosis Vaccine Initiative Team (SATVI)
    PLoS One. 2012; 7(10): e47597. PMID: 23112821 PMCID: PMC3480404
  11. A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guérin immunisation in children and adults.
    Ritz N, Strach M, Yau C, Dutta B, Tebruegge M, Connell TG, Hanekom WA, Britton WJ, Robins-Browne R, Curtis N.
    PLoS One. 2012;7(7):e37535. PMID: 22829867 PMCID: PMC3400612
  12. Moxifloxacin population pharmacokinetics in patients with pulmonary tuberculosis and the effect of intermittent high-dose rifapentine.
    Zvada SP, Denti P, Geldenhuys H, Meredith S, van As D, Hatherill M, Hanekom W, Wiesner L, Simonsson US, Jindani A, Harrison T, McIlleron HM.
    Antimicrob Agents Chemother. 2012 Aug;56(8):4471-3. PMID: 22585223 PMCID: PMC3421597
  13. A Phase IIa Trial of the New Tuberculosis Vaccine, MVA85A, in HIV- and/or Mycobacterium tuberculosis–infected Adults
    Thomas J. Scriba, Michele Tameris, Erica Smit, Linda van der Merwe, E. Jane Hughes, Blessing Kadira, Katya Mauff, Sizulu Moyo, Nathaniel Brittain, Alison Lawrie, Humphrey Mulenga, Marwou de Kock, Lebohang Makhethe, Esme Janse van Rensburg, Sebastian Gelderbloem, Ashley Veldsman, Mark Hatherill, Hendrik Geldenhuys, Adrian V. S. Hill, Anthony Hawkridge, Gregory D. Hussey, Willem A. Hanekom, Helen McShane, Hassan Mahomed
    Am J Respir Crit Care Med. 2012 Apr 1; 185(7): 769–778. PMID: 22281831 PMCID: PMC3326425
  14. HIV-1 infection alters CD4+ memory T-cell phenotype at the site of disease in extrapulmonary tuberculosis.
    Matthews K, Ntsekhe M, Syed F, Scriba T, Russell J, Tibazarwa K, Deffur A,Hanekom W, Mayosi BM, Wilkinson RJ, Wilkinson KA.
    Eur J Immunol. 2012 Jan;42(1):147-57. PMID: 22215422 PMCID: PMC3298896
  15. Optimization of a whole blood intracellular cytokine assay for measuring innate cell responses to mycobacteria.
    Shey MS, Hughes EJ, de Kock M, Barnard C, Stone L, Kollmann TR, Hanekom WA, Scriba TJ.
    J Immunol Methods. 2012 Feb 28;376(1-2):79-88. PMID: 22155193 PMCID: PMC3273614
  16. Human MAIT and CD8αα cells develop from a pool of type-17 precommitted CD8+ T cells
    Lucy J. Walker, Yu-Hoi Kang, Matthew O. Smith, Hannah Tharmalingham, Narayan Ramamurthy, Vicki M. Fleming, Natasha Sahgal, Alistair Leslie, Ye Oo, Alessandra Geremia, Thomas J. Scriba, Willem A. Hanekom, Georg M. Lauer, Olivier Lantz, David H. Adams, Fiona Powrie, Eleanor Barnes, Paul Klenerman
    Blood. 2012 Jan 12; 119(2): 422–433. PMID: 22086415 PMCID: PMC3257008
  17. Identification of antigens specific to non-tuberculous mycobacteria: the Mce family of proteins as a target of T cell immune responses.
    Checkley AM, Wyllie DH, Scriba TJ, Golubchik T, Hill AV, Hanekom WA, McShane H.
    PLoS One. 2011;6(10):e26434. PMID: 22046285 PMCID: PMC3201954
  18. Timing of antiretroviral therapy for HIV-1 infection and tuberculosis.
    Havlir DV, Kendall MA, Ive P, Kumwenda J, Swindells S, Qasba SS, Luetkemeyer AF, Hogg E, Rooney JF, Wu X, Hosseinipour MC, Lalloo U, Veloso VG, Some FF, Kumarasamy N, Padayatchi N, Santos BR, Reid S, Hakim J, Mohapi L, Mugyenyi P, Sanchez J, Lama JR, Pape JW, Sanchez A, Asmelash A, Moko E, Sawe F, Andersen J, Sanne I; AIDS Clinical Trials Group Study A5221.
    N Engl J Med. 2011 Oct 20;365(16):1482-91. PMID: 22010914 PMCID: PMC3327101
  19. CD4 T cell depletion at the cervix during HIV infection is associated with accumulation of terminally differentiated T cells.
    Gumbi PP, Jaumdally SZ, Salkinder AL, Burgers WA, Mkhize NN, Hanekom W, Coetzee D, Williamson A, Passmore JS.
    J Virol. 2011 Dec;85(24):13333-41. PMID: 21994461 PMCID: PMC3233181
  20. Immune activation in the female genital tract during HIV infection predicts mucosal CD4 depletion and HIV shedding.
    Jaspan HB, Liebenberg L, Hanekom W, Burgers W, Coetzee D, Williamson AL, Little F, Myer L, Coombs RW, Sodora D, Passmore JA.
    J Infect Dis. 2011 Nov 15;204(10):1550-6. PMID:21940422 PMCID:PMC3192190
  21. Association of human TLR1 and TLR6 deficiency with altered immune responses to BCG vaccination in South African infants.
    Randhawa AK, Shey MS, Keyser A, Peixoto B, Wells RD, de Kock M, Lerumo L, Hughes J, Hussey G, Hawkridge A, Kaplan G, Hanekom WA, Hawn TR; South African Tuberculosis Vaccine Initiative Team.
    PLoS Pathog. 2011 Aug;7(8):e1002174. PMID: 21852947 PMCID: PMC3154845
  22. Functional capacity of Mycobacterium tuberculosis-specific T cell responses in humans is associated with mycobacterial load.
    Day CL, Abrahams DA, Lerumo L, Janse van Rensburg E, Stone L, O'rie T, Pienaar B, de Kock M, Kaplan G, Mahomed H, Dheda K, Hanekom WA.
    J Immunol. 2011 Sep 1;187(5):2222-32. PMID: 21775682 PMCID: PMC3159795
  23. Predominance of interleukin-22 over interleukin-17 at the site of disease in human tuberculosis.
    Matthews K, Wilkinson KA, Kalsdorf B, Roberts T, Diacon A, Walzl G, Wolske J, Ntsekhe M, Syed F, Russell J, Mayosi BM, Dawson R, Dheda K, Wilkinson RJ, Hanekom WA, Scriba TJ.
    Tuberculosis (Edinb). 2011 Nov;91(6):587-93. PMID: 21767990 PMCID: PMC3220762
  24. The tuberculin skin test versus QuantiFERON TB Gold® in predicting tuberculosis disease in an adolescent cohort study in South Africa.
    Mahomed H, Hawkridge T, Verver S, Abrahams D, Geiter L, Hatherill M, Ehrlich R, Hanekom WA, Hussey GD.
    PLoS One. 2011 Mar 29;6(3):e17984. Erratum in: PLoS One. 2011;6(6). PMID: 21479236 PMCID: PMC3066222
  25. HIV-specific gag responses in early infancy correlate with clinical outcome and inversely with viral load.
    Nqoko B, Day CL, Mansoor N, De Kock M, Hughes EJ, Hawkridge T, Kaplan G, Boom WH, Hussey GD, Hanekom WA.
    AIDS Res Hum Retroviruses. 2011 Dec;27(12):1311-6. PMID: 21476948 PMCID: PMC3227240
  26. Induction of Granulysin and Perforin Cytolytic Mediator Expression in 10-Week-Old Infants Vaccinated with BCG at Birth
    Patricia L. Semple, Marcia Watkins, Virginia Davids, Alan M. Krensky, Willem A. Hanekom, Gilla Kaplan, Stanley Ress
    Clin Dev Immunol. 2011; 2011: 438463. PMID: 21234358 PMCID: PMC3018618
  27. Higher human CD4 T cell response to novel Mycobacterium tuberculosis latency associated antigens Rv2660 and Rv2659 in latent infection compared with tuberculosis disease.
    Govender L, Abel B, Hughes EJ, Scriba TJ, Kagina BM, de Kock M, Walzl G, Black G, Rosenkrands I, Hussey GD, Mahomed H, Andersen P, Hanekom WA.
    Vaccine. 2010 Dec 10;29(1):51-7. PMID: 20974305 PMCID: PMC3376751
  28. Novel application of Ki67 to quantify antigen-specific in vitro lymphoproliferation
    Andreia Soares, Lerisa Govender, Jane Hughes, Wendy Mavakla, Marwou de Kock, Charlene Barnard, Bernadette Pienaar, Esme Janse van Rensburg, Gail Jacobs, Gloria Khomba, Lynnette Stone, Brian Abel, Thomas J. Scriba, Willem A. Hanekom
    J Immunol Methods. 2010 Oct 31; 362(1-2): 43–50. PMID: 20800066 PMCID: PMC2989440
  29. Specific T Cell Frequency and Cytokine Expression Profile Do Not Correlate with Protection against Tuberculosis after Bacillus Calmette-Guérin Vaccination of Newborns
    Benjamin M. N. Kagina, Brian Abel, Thomas J. Scriba, Elizabeth J. Hughes, Alana Keyser, Andreia Soares, Hoyam Gamieldien, Mzwandile Sidibana, Mark Hatherill, Sebastian Gelderbloem, Hassan Mahomed, Anthony Hawkridge, Gregory Hussey, Gilla Kaplan, Willem A. Hanekom, and other members of the South African Tuberculosis Vaccine Initiative
    Am J Respir Crit Care Med. 2010 Oct 15; 182(8): 1073–1079. PMID: 20558627 PMCID:PMC2970848
  30. Single nucleotide polymorphisms in toll-like receptor 6 are associated with altered lipopeptide- and mycobacteria-induced interleukin-6 secretion.
    Shey MS, Randhawa AK, Bowmaker M, Smith E, Scriba TJ, de Kock M, Mahomed H, Hussey G, Hawn TR, Hanekom WA.
    Genes Immun. 2010 Oct;11(7):561-72. PMID: 20445564 PMCID: PMC3518443
  31. Structured approaches for the screening and diagnosis of childhood tuberculosis in a high prevalence region of South Africa.
    Hatherill M, Hanslo M, Hawkridge T, Little F, Workman L, Mahomed H, Tameris M, Moyo S, Geldenhuys H, Hanekom W, Geiter L, Hussey G.
    Bull World Health Organ. 2010 Apr;88(4):312-20. PMID: 20431796 PMCID: PMC2855594
  32. CD4 and CD8 T-cell responses to mycobacterial antigens in African children.
    Tena-Coki NG, Scriba TJ, Peteni N, Eley B, Wilkinson RJ, Andersen P, Hanekom WA, Kampmann B.
    Am J Respir Crit Care Med. 2010 Jul 1;182(1):120-9. PMID: 20224065 PMCID: PMC2902756
  33. Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activity.
    Joosten SA, van Meijgaarden KE, van Weeren PC, Kazi F, Geluk A, Savage ND, Drijfhout JW, Flower DR, Hanekom WA, Klein MR, Ottenhoff TH.
    PLoS Pathog. 2010 Feb 26;6(2):e1000782. PMID: 20195504 PMCID: PMC2829052
  34. The Novel Tuberculosis Vaccine, AERAS-402, Induces Robust and Polyfunctional CD4+ and CD8+ T Cells in Adults
    Brian Abel, Michele Tameris, Nazma Mansoor, Sebastian Gelderbloem, Jane Hughes, Deborah Abrahams, Lebohang Makhethe, Mzwandile Erasmus, Marwou de Kock, Linda van der Merwe, Anthony Hawkridge, Ashley Veldsman, Mark Hatherill, Giulia Schirru, Maria Grazia Pau, Jenny Hendriks, Gerrit Jan Weverling, Jaap Goudsmit, Donata Sizemore, J. Bruce McClain, Margaret Goetz, Jacqueline Gearhart, Hassan Mahomed, Gregory D. Hussey, Jerald C. Sadoff, Willem A. Hanekom
    Am J Respir Crit Care Med. 2010 Jun 15; 181(12): 1407–1417. PMID: 20167847 PMCID:PMC2894413
  35. Comparison of polyclonal expansion methods to improve the recovery of cervical cytobrush-derived T cells from the female genital tract of HIV-infected women.
    Bere A, Denny L, Hanekom W, Burgers WA, Passmore JA.
    J Immunol Methods. 2010 Mar 31;354(1-2):68-79. PMID: 20149794 PMCID: PMC2854893
  36. Modified vaccinia Ankara-expressing Ag85A, a novel tuberculosis vaccine, is safe in adolescents and children, and induces polyfunctional CD4+ T cells.
    Scriba TJ, Tameris M, Mansoor N, Smit E, van der Merwe L, Isaacs F, Keyser A, Moyo S, Brittain N, Lawrie A, Gelderbloem S, Veldsman A, Hatherill M, Hawkridge A, Hill AV, Hussey GD, Mahomed H, McShane H, Hanekom WA.
    Eur J Immunol. 2010 Jan;40(1):279-90. doi: 10.1002/eji.200939754. Erratum in: Eur J Immunol. 2011 May;41(5):1501. PMID: 20017188 PMCID: PMC3044835
  37. Comparison of mantoux and tine tuberculin skin tests in BCG-vaccinated children investigated for tuberculosis.
    Pan W, Matizirofa L, Workman L, Hawkridge T, Hanekom W, Mahomed H, Hussey G, Hatherill M.
    PLoS One. 2009 Nov 30;4(11):e8085. PMID: 19956612 PMCID: PMC2779491
  38. Two loci control tuberculin skin test reactivity in an area hyperendemic for tuberculosis.
    Cobat A, Gallant CJ, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Jaïs JP, Boland-Auge A, van Helden P, Casanova JL, Abel L, Hoal EG, Schurr E, Alcaïs A.
    J Exp Med. 2009 Nov 23;206(12):2583-91. PMID: 19901083 PMCID: PMC2806605
  39. HIV-1 Infection Impairs the Bronchoalveolar T-Cell Response to Mycobacteria
    Barbara Kalsdorf, Thomas J. Scriba, Kathryn Wood, Cheryl L. Day, Keertan Dheda, Rodney Dawson, Willem A. Hanekom, Christoph Lange, Robert J. Wilkinson
    Am J Respir Crit Care Med. 2009 Dec 15; 180(12): 1262–1270. PMID: 19797156 PMCID: PMC2796736
  40. Impact of human immunodeficiency virus 1 infection and inflammation on the composition and yield of cervical mononuclear cells in the female genital tract.
    Nkwanyana NN, Gumbi PP, Roberts L, Denny L, Hanekom W, Soares A, Allan B, Williamson AL, Coetzee D, Olivier AJ, Burgers WA, Passmore JA.
    Immunology. 2009 Sep;128(1 Suppl):e746-57. PMID: 19740336 PMCID: PMC2753906
  41. Dissection of regenerating T-Cell responses against tuberculosis in HIV-infected adults sensitized by Mycobacterium tuberculosis.
    Wilkinson KA, Seldon R, Meintjes G, Rangaka MX, Hanekom WA, Maartens G, Wilkinson RJ.
    Am J Respir Crit Care Med. 2009 Oct 1;180(7):674-83. PMID: 19628776 PMCID: PMC4176738
  42. Delaying BCG vaccination from birth to 10 weeks of age may result in an enhanced memory CD4 T cell response.
    Kagina BM, Abel B, Bowmaker M, Scriba TJ, Gelderbloem S, Smit E, Erasmus M, Nene N, Walzl G, Black G, Hussey GD, Hesseling AC, Hanekom WA.
    Vaccine. 2009 Sep 4;27(40):5488-95. PMID: 19616494 PMCID: PMC2745558
  43. Immunogenicity of novel DosR regulon-encoded candidate antigens of Mycobacterium tuberculosis in three high-burden populations in Africa.
    Black GF, Thiel BA, Ota MO, Parida SK, Adegbola R, Boom WH, Dockrell HM, Franken KL, Friggen AH, Hill PC, Klein MR, Lalor MK, Mayanja H, Schoolnik G, Stanley K, Weldingh K, Kaufmann SH, Walzl G, Ottenhoff TH; GCGH Biomarkers for TB Consortium.
    Clin Vaccine Immunol. 2009 Aug;16(8):1203-12. PMID: 19553548 PMCID: PMC2725533